Design and Assessment of Species-Level qPCR Primers Targeting Comammox
Published PCR primers targeting the ammonia monooxygenase gene (amoA) were applied to wedding bands in fresno samples from activated sludge systems operated with low dissolved oxygen (DO) to quantify total and clade-level Nitrospira that perform complete ammonium oxidation (comammox); however, we found these existing primers resulted in significant